FIG 7.

PLD1 silencing prevents TNP-induced autophagy and decreases in HIV p24 release. Mock- and HIV-infected macrophages (A to C) and CD4⁺ T cells (D to F) transfected with PLD1 siRNA (siPLD1) or scrambled siRNA (siNS) were exposed to vehicle, 400 μg ml⁻¹ RBC-NP (R), or 400 μg ml⁻¹ TNP (T) for 4 h, washed three times with PBS, and then incubated for a further 24 h. n = 4. (A and D) (Bottom) Representative western blots of PLD1. (Top) Densitometric analysis of blots. (B and E) (Top) Representative western blots of LC3B isoforms and SQSTM1. (Bottom) Densitometric analysis of blots. (C and F) Enzyme-linked immunosorbent assays were performed for HIV p24 antigen in the supernatant.