FIG 2.
The type III secreted effector Map triggers an increase in pERK levels in HeLa and Caco-2 cells. (A) Screening for effects on pERK levels by EPEC strains mutated at different LEE-borne effector genes. HeLa cells were infected with the indicated mutant strains (Table S1), and pERK levels were analyzed, as for Fig. 1. (B and C) Mapwt translocation induces pERK in HeLa and CaCo-2 cells, and EspH counteracts this effect. Cells were infected with the indicated EPEC strains (Table S1), and pERK levels in the infected cells were analyzed, as for Fig. 1. Results are means and SE from 3 independent experiments. ***, P < 0.0005; **, P < 0.005; *, P < 0.05; ns, nonsignificant (P > 0.05).