. 2020 Sep 15;11(5):e02219-20. doi: 10.1128/mBio.02219-20

TABLE 4.

Primers used in this study

purposePrimer purpose	Primer name	Primer sequence	PCR product size (bp)
pJIR750virSi construction	virS-293\|294a-IBS	AAAAAAGCTTATAATTATCCTTATCTACCGTAAGCGTGCGCCCAGATAGGGTG	350
	virS-293\|294a-EBS1d	CAGATTGTACAAATGTGGTGATAACAGATAAGTCGTAAGCATTAACTTACCTTTCTTTGT
	virS-293\|294a-EBS2	TGAACGCAAGTTTCTAATTTCGGTTGTAGATCGATAGAGGAAAGTGTCT
	EBS universal	CGAAATTAGAAACTTGC GTTCAGTAAAC
Screen for intron insertion in virS (virS RT-PCR)	virSKOF	TAAGTCAATTTAGCCCTAAGAAAA	376
Screen for intron insertion in virS (virS RT-PCR)	virSKOR	CGAAAC TTTAAACATCTAACAACCA	376
Screen for intron insertion in agrB (agrB RT-PCR)	NagrBKOF	TGGAACTTATGCTCTAATACAAACA	536
Screen for intron insertion in agrB (agrB RT-PCR)	agrBKOR	AATCTATAGTTTTTAACAATATATTT	536
cpb qRT-PCR	cpbF	GCGAATATGCTGAATCATCTA	196
cpb qRT-PCR	cpbR	GCAGGAACATTAGTATATCTTC	196
virS qRT-PCR	qvirSF	CATAGCCTGTATTGAAGGAAATAAC	229
virS qRT-PCR	qvirSR	TGTGCAGATATCAAAGTACTCA	229
virR qRT-PCR	qvirRF	CCTTTGAGACAGGAGAGGATCTA	240
virR qRT-PCR	qvirRR	CCTGCTCTTGTAGCTCCTTAAAT	240
agrD qRT-PCR	qagrDF	GCTGCATTAACAACAGTAGTTGC	76
agrD qRT-PCR	qagrDR	GTTCCTCTGGTTGGTGTGTAAA	76
virR/S operon sequencing	VirR/SseqF	GTCTCAAAGATCTAGTAAAATGGGA	838
	VirR/SseqR	GCCCTTATTATTAAGCTCCTTTTCT	838
	VirR/Sseq1	CAGGTTACAGCTTGTGTAGAAAATA	912
	VirR/Sseq1R	CCTTAAAGGCATATCCAAATATAAC	912
	VirR/Sseq2	CAATATAAAATGTATTATGATCTC	1,050
	VirR/Sseq2R	GGAATGAGCATTTTTAATATGAATT	1,050
	VirR/Sseq3	GACAAGCTAAACTTAGGATT	813
	VirR/Sseq3R	TTCCATTTACCTGAATTAACTCACT	813
virR/S complementation	VirR/ScompF	CCGGGGATCCGAAAGTGGATATGCACTAGGAAC	2,494
virR/S complementation	VirR/ScompR	ATGCCTGCAGTGCAAAGCTTAAAACTGTAACTGTA	2,494
virR/S-His tag complementation	VirR/ShisR	ATGCCTGCAGTTAATGATGATGATGATGATGGGCTTCTTTTTCTTGATTTATAGG	2,249