Table 1.

Statistics analysis of transformation rate and regeneration ability in transgenic wheat.

Transformed plasmid	PWMB003(Control 1)	pWMB003-TaTCP-1 (OE)	pAHC25(Control 2)	pAHC25-TaTCP-1 RNAi (RNAi)
No. of bombardment callus	505	2020	1439	2800
No. of T0 regenerate plants	29	146	195	350
No. of T0 positive plants	4	17	19	34
Transformation rate	0.79%	0.84%	1.32%	1.21%
No. of T0 callus	90	408	712	1248
No. of T0 regenerate plants	19	145	1056	789
Regeneration rate of T0	21.11%	35.54%	148.31	63.22%
No. of T1 positive plants	3	8	39	26
No. of T1 callus	85	220	936	702
No. of T1 regenerate plants	18	79	1401	436
Regeneration rate of T1	21.18%	35.91%	149.68%	62.11%
No. of T2 positive plants	3	6	45	28
No. of T2 callus	90	216	942	758
No. of T2 regenerate plants	20	85	1425	452
Regeneration rate of T2	22.22%	39.35%	151.27%	59.63%
No. of T3 positive plants	6	22	625	186

Regeneration rate: Number of resistant regeneration plantlets/Number of calli culturedⅹ100.