Table 1.
RT-PCR targeted genes and primer sequences with their expected amplicon size.
| Gene | Primer pair | Primer sequence (5′ ⟶ 3′) | Amplicon size (bp) |
|---|---|---|---|
| aflR | Forward | CCGTCAGACAGCCACTGGACACGG | 300 |
| Reverse | TGACCCACCTCTTCCCCCACG | ||
|
| |||
| aflS | Forward | GAACGCTGATTGCCAATGCC | 1256 |
| Reverse | CGGTCAGGATGTTACTAAGC | ||
|
| |||
| aflD | Forward | ACCGCTACGCCGGCACTCTCGGAC | 400 |
| Reverse | GTTGGCCGCCAGCTCTGACACTC | ||
|
| |||
| aflP | Forward | GTGGACGGACCTAGTCCGACATCC | 624 |
| Reverse | GTCGGCGCCACGCACTGGGTTGGG | ||
|
| |||
| aflQ | Forward | TTAAGGCAGCGGAATACAAG | 599 |
| Reverse | GACGCCCAAAGCCGAACACAAA | ||
|
| |||
| tub1 | Forward | GCTTTCTGGCAAACCATCTC | 1198 |
| Reverse | GGTCGTTCATGTTGCTCTCA | ||