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. 2020 Sep 5;2020:2194090. doi: 10.1155/2020/2194090

Figure 3.

Figure 3

The effects induced by the HSP70 inhibitor could be blocked by the p38 inhibitor. (a) Expression levels of HSP70, p38, and p-p38 were analyzed in the cells treated with 0, 5, 10, and 20 μM Apoptozole. (b) CCK-8 assay was used to measure cell viability in HUVEC under treatments with Apoptozole and/or SB203580. (c) ELISA was used to detect the productions of IL-1β, IL-6, and TNF-α after treatments. (d, e) Endothelial permeability was measured using TEER assay and FITC-Dextrans. (f) Expression levels of E-cadherin, occludin, ZO-1, p38, and p-p38 were measured by western blot. ∗P < 0.05; ∗∗∗P < 0.001 vs. control; +++P < 0.001 vs. Apoptozole.