FIGURE 6.

JQ1 enhances autophagy and restores autophagic flux after SCI. (A–D) Primary cortical neurons were treated with 200 nM JQ1 for 6 h, the level of LC3, Atg5, and Beclin-1 in each group of neuron were evaluated by western blotting and statistically analyzed, n = 5. (E,F) Primary cortical neurons were treated with Baf-A1 (100 nM) and JQ1 (200 nM) for 6 h, the level of LC3 in each group of neuron were detected by western blotting and statistically analyzed, n = 5. (G–I) Western blotting and quantification of p62 and LC3 expression in each group of mice at 3 days after SCI, n = 5. (J,K) Representative co-staining for LC3 (red) and NeuN (green) in each group of mice at 3 days after SCI, n = 5. Scale bar = 50 μm, scale bar (enlarged) = 10 μm. (L,M) Primary cortical neurons were treated with TBHP (50 μM) and JQ1 (200 nM) for 6 h, the co-localization of LC3 (green) and Lamp2 (red) were captured by immunofluorescence staining and quantitatively analyzed, n = 10. Scale bar = 20 μm, scale bar (enlarged) = 5 μm. GAPDH was the loading control. *P < 0.05, **P < 0.01, ***P < 0.001. Data were presented as means ± SD.