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. 2020 Sep 2;11:559627. doi: 10.3389/fphar.2020.559627

Figure 4.

Figure 4

The effect of ketamine on synaptic-level proteins in hippocampal synaptosomes. (A) Representative Western Blot images of synaptic-level protein expression in the hippocampus after ketamine incubations on synaptosomes derived from corticosterone (CORT)-treated animals. Ladder presented is Spectra Multicolor High Range Protein Ladder (Thermofisher, #26625). (B) Effects of ketamine incubations on postsynaptic density-95 (PSD-95). CORT decreased this expression while all ketamine incubations increased these levels. (C) Effects of ketamine incubations on mammalian target of rapamycin (mTOR). All concentrations of ketamine increased mTOR levels above CORT-treated animals. (D) Effects of ketamine incubations compared to those of reelin on p-mTOR. Only 10nм of ketamine increased p-mTOR levels above CORT-treated animals. (E) Effects of ketamine incubations on the active ratio of mTOR. All data are expressed as mean±SD. K50, 50nм ketamine; K100, 100nм ketamine; K500, 500nм ketamine. #p < 0.05, ##p < 0.01 vs. vehicle, *p < 0.05, **p < 0.01, ***p < 0.001 vs. CORT.