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. 2020 Sep 2;11:559627. doi: 10.3389/fphar.2020.559627

Figure 5.

Figure 5

The effect of ketamine on synaptic-level proteins in cerebellar synaptosomes. (A) Representative Western Blot images of synaptic-level protein expression in the cerebellum after ketamine incubations on synaptosomes from corticosterone (CORT)-treated animals. Ladder presented is Spectra Multicolor High Range Protein Ladder (Thermofisher, #26625). (B) Effects of ketamine incubations on levels of postsynaptic density-95 (PSD-95). All levels stayed the same. (C) Effects of ketamine incubations on levels of mammalian target of rapamycin (mTOR). All concentrations of ketamine increased the levels above vehicle and CORT. (D) Effects of ketamine on levels of p-mTOR. All concentrations were significantly different from both vehicle and CORT. (E) Effects of ketamine on the active ratio of mTOR. All data are expressed as mean±SD. K5, 5nм ketamine; K10, 10nм ketamine; K50, 50nм ketamine. #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. vehicle, *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. CORT.