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. 2020 Aug 7;13:7887–7896. doi: 10.2147/OTT.S238189

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© 2020 Zhang et al.

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Role of TUG1 knockdown in human AML cells in vitro. (A–G) HL-60 and KG-1 cells were transfected with TUG1 siRNA (si-TUG1) or its negative control si-NC. (A) RT-qPCR analyzed TUG1 expression level after transfection. (B and C) Cell viability was measured by MTT assay. (D and E) The glucose consumption and lactate production in the cell supernatant of transfected cells were measured by commercial kits. (F) Western blot assay examined expression of hexokinase 2 (HK2) after transfection. (G) Apoptotic rate was determined using flow cytometry. The error bars represent the mean±SEM of three independent experiments. **P<0.01, and ***P<0.001 compared with the si-NC group.