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. 2020 Aug 7;13:7887–7896. doi: 10.2147/OTT.S238189

Figure 3.

Figure 3

The regulatory effect of TUG1 on miR-185 expression. (A) The miR-185 binding sites were shown on the wild type of human TUG1 (TUG1-WT) according to Starbase. The corresponding sequence in the mutated version (TUG1-MUT) was shown as well. (B and C) Luciferase reporter assay detected the luciferase activities of TUG1-WT and TUG1-MUT in HL-60 and KG-1 cells transfected with miR-185/NC mimic (miR-185/NC). (D and E) RNA immunoprecipitation (RIP) assay was performed to identify TUG1 levels in HL-60 and KG-1 cells transfected with miR-185/NC. (F and G) Expression level of miR-185 was detected in HL-60 and KG-1 cells after transfection of si-TUG1, pcDNA-TUG1 (TUG1), or matched controls. The error bars represent the mean±SEM of three independent experiments. ***P<0.001 compared with control groups (miR-NC, si-NC, or Vector).