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. 2020 Aug 7;13:7887–7896. doi: 10.2147/OTT.S238189

Figure 5.

Figure 5

Role of miR-185 overexpression in human AML cells in vitro. (AG) HL-60 and KG-1 cells were transfected with miR-185/NC. After transfection, (A) RT-qPCR determined miR-185 expression level, (B and C) MTT assay measured cell viability, (D and E) commercial kits measured glucose consumption and lactate production, (F) Western blot assay examined HK2 expression, and (G) flow cytometry determined apoptotic rate. The error bars represent the mean±SEM of three independent experiments. *P<0.05, **P<0.01, and ***P<0.001 compared with the miR-NC group.