Figure 6.
Influence of miR-185 on the role of TUG1 knockdown in human AML cells in vitro. (A–J) HL-60 and KG-1 cells were transfected with si-TUG1/NC or co-transfected with si-TUG1 and anti-miR-185/NC. After transfection, (A and B) cell viability, (C–F) glucose consumption and lactate production, (G and H) HK2 expression, and (I and J) apoptotic rate were measured by MTT assay, commercial kits, Western blot, and flow cytometry, respectively. The error bars represent the mean±SEM of three independent experiments. *P<0.05, **P<0.01, and ***P<0.001 compared with controls (si-NC or si-TUG1+anti-miR-NC).