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. 2020 Sep 3;13:8783–8794. doi: 10.2147/OTT.S258450

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© 2020 Zhou et al.

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Overexpression of RIG-I by lentivirus system in mouse peritoneal macrophage promoted M1 polarization in vitro. (A) Identification of the purity of isolated peritoneal macrophages in mice by FACS. (B) The mouse peritoneal macrophages were collected and transfected with RIG-I lentivirus for 72h. The transfection efficiency of RIG-I was observed by fluorescence microscope ×400. (C) FACS assay was used to evaluate expression of the biomarker CD16/32 and CD11c associated with M1.