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. 2019 Nov 19;12(7):530–542. doi: 10.1093/jmcb/mjz103

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© The Author(s) (2019). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Overexpression of Cdc42se1 in PGCs leads to mislocalization. (A) Cdc42se1-RFP-UTR-nos3 mRNA was injected into 4-cell embryos of Tg1 kop:EGFP-UTR-nos3 line. (B) The number of mislocalized PGCs in embryos injected with RFP or Cdc42se1-RFP-UTR-nos3 mRNA. (C and D) Representative images of PGCs in embryos injected with RFP or Cdc42se1-RFP-UTR-nos3 at prim 5 stage. (E and F) The magnified images as indicated in D. Arrows indicate the mislocalized PGCs, and asterisks indicate the dead or transforming PGCs. ****P < 0.0001. Scale bar, 100 μm (E and F) and 20 μm (D).