Table 3.
List of engineered strains of DSM 7029 used in this study
| Classification | Strain | Relevant genotype/property | Source |
|---|---|---|---|
| Redox gene overexpression | H7029-1 | Epothilone C/D high-yield strain | This study |
| H7029-2 | H7029-1-epoK | This study | |
| H7029-3 | H7029-1-epoK-fdx_0135 | This study | |
| H7029-4 | H7029-1-epoK-fdx_2170 | This study | |
| H7029-5 | H7029-1-epoK-fdx_2730 | This study | |
| H7029-6 | H7029-1-epoK-fdx_4560 | This study | |
| H7029-7 | H7029-1-epoK-fdx_5185 | This study | |
| H7029-8 | H7029-1-epoK-fdx_6105 | This study | |
| H7029-9 | H7029-1-epoK-fdx_S4580 | This study | |
| H7029-10 | H7029-1-epoK-fdx_A6445 | This study | |
| H7029-11 | H7029-1-epoK-fdr_0130 | This study | |
| H7029-12 | H7029-1-epoK-fdr_7100 | This study | |
| H7029-13 | H7029-1-epoK-fdr_S2240 | This study | |
| H7029-14 | H7029-1-epoK-fdx_0135-fdr_0130 | This study | |
| H7029-15 | H7029-1-epoK-fdx_0135-fdr_7100 | This study | |
| H7029-16 | H7029-1-epoK-fdx_S4580-fdr_S2240 | This study | |
| Whole-cell biotransformation | 7029-1 |
DSM 7029 derivate, harbouring pEOK101, DSM 7029-empty vector |
This study |
| 7029-2 |
DSM 7029 derivate, harbouring pEOK102, DSM 7029-epoK |
This study | |
| 7029-3 |
DSM 7029 derivate, harbouring pEOK10, DSM 7029-epoK-fdx_0135 |
This study | |
| 7029-4 |
DSM 7029 derivate, harbouring pEOK109, DSM 7029-epoK-fdx_S4580 |
This study | |
| 7029-5 |
DSM 7029 derivate, harbouring pEOK11, DSM 7029-epoK-fdr_0130 |
This study | |
| 7029-6 |
DSM 7029 derivate, harbouring pEOK112, DSM 7029-epoK-fdr_7100 |
This study | |
| 7029-7 |
DSM 7029 derivate, harbouring pEOK113, DSM 7029-epoK-fdr_S2240 |
This study | |
| 7029-8 |
DSM 7029 derivate, harbouring pEOK114, DSM 7029-epoK-fdx_0135-fdr_0130 |
This study | |
| 7029-9 |
DSM 7029 derivate, harbouring pEOK115, DSM 7029-epoK-fdx_0135-fdr_7100 |
This study | |
| 7029-10 |
DSM 7029 derivate, harbouring pEOK116, DSM 7029-epoK-fdx_S4580-fdr_S2240 |
This study | |
| Redox gene knock-out | JH01 | H7029-14 derivate, fdx_0135 knock-out | This study |
| JH02 | H7029-14 derivate, fdr_0130 knock-out | This study | |
| JH03 | H7029-12 derivate, fdr_7100 knock-out | This study |