Table 4.
Effects of Crude Extract and Fractions on Biochemical Parameters Against Paracetamol-Induced Hepatotoxicity in Mice
| Serum Enzyme Levels | |||||
|---|---|---|---|---|---|
| Treatment Group |
AST (U/L) | ALT(U/L) | ALP (U/L) | Total Bilirubin (mg/dl) | Total Protein (mg/dl) |
| Normal(5% DMSO) | 105.83±3.46 | 56.83±2.91 | 219.66±7.12 | 0.45±0.04 | 8.48±0.93 |
| Paracetamol (250mg/kg) | 313.50±13.75a | 164.06±6.95a | 506.72±16.38a | 1.27±0.07a | 4.68±0.48b |
| Silymarin (100mg/kg) | 161.50±5.60*** | 82.33±3.49*** | 268.83±6.26*** | 0.70±0.08*** | 8.00±0.57** |
| Crude(100mg/kg) | 250.55±9.49*** | 122.6±5.48*** | 381.50±10.86*** | 1.07±0.05 | 6.67±0.45 |
| Crude(200mg/kg) | 214.46±5.91*** | 103.43±6.87*** | 325.28±11.47*** | 0.99±0.06* | 6.91±0.37 |
| Crude (400mg/kg) | 183.42±5.86*** | 91.10±4.26*** | 303.98±8.47*** | 0.72±0.04*** | 7.90±0.55* |
| Aqueous(100mg/kg) | 242.03±7.35*** | 119.67±4.66*** | 402.04±9.28*** | 1.02±0.03 | 6.73±0.84 |
| Aqueous(200mg/kg) | 205.87±6.05*** | 98.72±5.08*** | 360.12±8.05*** | 0.96±0.0.07** | 7.16±0.45 |
| Aqueous (400mg/kg) | 197.65±5.68*** | 86.33±4.67*** | 317.21±10.72*** | 0.82±0.0.06*** | 7.86±0.37* |
| Chloroform(100mg/kg) | 256.47±8.14*** | 121.47±6.90*** | 416.32±12.72*** | 1.08±0.03** | 6.38±0.51 |
| Chloroform(200mg/kg) | 215.10±7.62*** | 104.57±5.48*** | 389.18±8.52*** | 0.98±0.05* | 6.93±0.49 |
| Chloroform(400mg/kg) | 196.55±4.84*** | 96.42±4.34*** | 321.50±9.35*** | 0.86±0.04*** | 7.29±0.68 |
Notes: N= 6; Results were expressed in mean ± SEM. aP<0.001;bP<0.01 Vs Normal group animals; ***P<0.001,**P<0.01, *P<0.05 vs paracetamol group animals. Data were analyzed by One-Way ANOVA followed by Tukey-Kramer multiple comparison test.
Abbreviation: DMSO, dimethyl sulfoxide.