Figure 2.

The global transcriptome analysis on rat dorsal root ganglia (DRG) identifies the genes that are significantly altered in one day after plantar incision. The infiltration of capsaicin 30 min prior to surgical incision completely prevented incision-induced transcriptional changes in 99 of 126 (79%) genes; shown in a heat map (a). Each line represents a gene across three groups (n = 4/group). Shades of blue and orange indicate negative and positive z-scores, respectively. (b) Venn diagram showing altered functions associated with two distinct groups of genes. Both groups underwent plantar incision but one was pretreated with capsaicin (inci + cap) and the other was not (Inci). Values in parentheses indicate z-score where negative and positive values predict inhibition and activation, respectively. (c) The differentially expressed genes from Inci + cap and Inci were compared by IPA. The top diseases and biological functions were related to “mortality morbidity” and “organismal death”. Dots indicate z-score <2.0.