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. 2020 Sep 15;21:66. doi: 10.1186/s12860-020-00307-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — SOX9 immunofluorescence staining in NT2/D1 cells after 48 h of culture. SOX9 (green) was primarily nuclear (nuclei marked by DAPI; magenta) in the control cells. EE2 treatment at both 10 nM and 100 nM led to a greater amount of SOX9 in the cytoplasm. Top panel shows SOX9, middle panel shows DAPI, bottom panel shows a merge. Scale bar 5 μm