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. 2020 Sep 15;36:31. doi: 10.1186/s42826-020-00065-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Analysis of tissue-specific Cre activity. a qRT-PCR analysis of relative Cre mRNA levels in various tissues. Total RNA prepared from VIPR2-ERT2CreERT2^Tg/+ adult mice was analyzed by real-time PCR and the relative Cre mRNA level in each tissue was compared to the level of the heart (n = 2). The blue line indicates the non-specific Cre mRNA level of’ the wild-type heart. Data are presented as mean ± SD. Only the level of the heart is statistically significant (p < 0.01). b Strategies for treating adult VIPR2:R26 compound heterozygotes with tamoxifen followed by LacZ staining. c Frozen sections of the heart were stained with X-gal followed by counterstaining with H&E briefly. 1, right atrium; 2 and 3, left atrium; 4, aortic valve; 5, right ventricle; 6, left ventricle. Original magnification, 40×; Right, 100× magnification of rectangles 1 to 6