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. 2020 Sep 15;19:181. doi: 10.1186/s12934-020-01440-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Analysis of hydrolysis products by HPLC. a The hydrolysis products of colloidal chitin with different concentration by recombinant ChiA for 12 h. 1: Standard of GlcNAc (peak a) and N, N′-diacetylchitobiose (peak b). 2–7: 10%, 15%, 25%, 30%, 35%, 40% colloidal chitin as substrate, respectively. b The hydrolysis products of N, N′-diacetylchitobiose by recombinant ChiA. 1: Standard of GlcNAc (peak a) and N, N′-diacetylchitobiose (peak b). 2, 3: The hydrolysis product of N, N′-diacetylchitobiose by recombinant ChiA at 0, 20 min, respectively. C The hydrolysis products of colloidal chitin by recombinant ChiA/BsNagZ. 1: Standard of GlcNAc (peak a) and N, N′-diacetylchitobiose (peak b). 2, 3: The hydrolysis product of colloidal chitin by recombinant ChiA for 12 h and then with recombinant BsNagZ for 0 (2), 20 min (3)