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Wound-induced spatiotemporal expression of IL-36α. qPCR (A) and western blotting (B,C) for IL-36α expression at the wound area from PWD 7–20 and normal mouse skin (control). (D–I) Immunohistochemistry of wounded and unwounded mouse skin paraffin sections to detect IL-36α in the wound area and wound edge at PWD 0 (D), 7 (E), 10 (F), 12 (G), 15 (H), and 20 (I) (n = 5, **p < 0.005, N, normal skin; PWD, post-wound day).
