FIGURE 3.
IL-36α promotes wound epithelialization and wound induced hair follicle neogenesis. A full-thickness wound of 1.5 × 1.5 cm2 square was made. A total of 50 μL of 1 ng/μL mrIL-36α dissolved in PBS or PBS only was injected into the healing wound (beneath the scab into the granulation tissue) from post-wound day (PWD) 7–14. (A) Wound model photographs of murine recombinant IL-36α (mrIL-36α)-injected group (n = 10) and control group (n = 10). (B) De novo hair follicles in skin stained for alkaline phosphatase activity at PWD 28. (C) De novo hair follicle counting in wild type mice after single dose of mrIL-36α (50 ng) compared to PBS control (**p < 0.005, n = 20).