FIGURE 6.

Verification of the changes in expression of some differentially expressed genes using real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. N = 3 biological repeats (3 mice)/age. Data were shown as mean ± SEM. (A,B) Expression of Igfbpl1 mRNA in the hippocampus (A) and Ostn mRNA in ACC (B) were significantly reduced in both 12- and 24-month old mice as compared to 2-month old mice. ^∗∗P < 0.01 versus the corresponding 2-month old mice by one-way ANOVA with repeated measures followed by post hoc Tukey test. (C,D) Expression of Klk6 mRNA in amygdala (C) and Defb1 mRNA in ACC (D) were significantly increased in 12- and 24-month old mice as compared to 2-month old mice. ^*P < 0.05, ^∗∗P < 0.01 versus 2-month old mice by one-way ANOVA with repeated measures followed by post hoc Tukey test.