Figure 5.

Aβ and H₂O₂ treatment both activated the SASP pathway and induced secretion of IL-1β in rat astrocytes. (A,B) mRNA levels of IL-1β, IL-6, and IL-8 in rat astrocytes treated with or without Aβ (A) or H₂O₂ (B) for 2 days. (C) Protein levels of pro-IL-1β and mature-IL-1β in rat astrocytes treated with different concentrations of Aβ (a) or H₂O₂ (b) for 2 days. (D,E) Quantification of (C). (F) ELISA quantification of the concentration of IL-1β in the supernatant of rat astrocytes treated with or without Aβ (300 ng/mL) or H₂O₂ (30 μM) for 2 days. Data indicate the mean values calculated from three independent experiments (±SD).