Table 3.
Association Between Serum Cytokine Levels and SNPs in Evaluation Cohort
| SNP | N | Mean Serum CCL5 Levels (pg/mL) | Mean Serum VEGF-A Levels (pg/mL) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Baseline | Day 21 | Quantitative Change | Increase (%) | Baseline | Day 21 | Quantitative Change | Increase (%) | ||
| CCL5 rs2280789 | |||||||||
| A/A | 20 | 67,125.2 | 66,218.1 | −1167.7 | 47.4 | 404.0 | 621.0 | 205.2 | 68.4 |
| A/G | 22 | 63,290.4 | 64,017.6 | 727.2 | 50.0 | 369.4 | 509.0 | 139.6 | 54.5 |
| G/G | 15 | 44,844.5 | 43,344.6 | −1499.9 | 46.7 | 251.4 | 324.1 | 72.7 | 66.7 |
| P | .010a | .008a | .97 | 1.00 | .14 | .062 | .34 | .65 | |
| Any A | 42 | 65,116.5 | 65,037.4 | −150.9 | 48.8 | 385.8 | 560.9 | 170.0 | 61.0 |
| G/G | 15 | 44,844.5 | 43,344.6 | −1499.9 | 46.7 | 251.4 | 324.1 | 72.7 | 66.7 |
| P | .003a | .009a | .82 | 1.00 | .13 | .024a | .23 | .76 | |
| CCL5 rs3817655 | |||||||||
| A/A | 20 | 67,125.2 | 66,218.1 | −1167.7 | 47.4 | 404.0 | 621.0 | 205.2 | 68.4 |
| A/T | 21 | 61,796.6 | 64,922.4 | 3125.8 | 52.4 | 381.9 | 525.8 | 143.9 | 52.4 |
| T/T | 16 | 47,958.0 | 43,449.1 | −4508.8 | 43.8 | 242.3 | 313.5 | 71.2 | 68.8 |
| P | .039a | .005a | .68 | .94 | .45 | .041a | .32 | .58 | |
| Any A | 41 | 64,396.0 | 65,537.9 | 1086.4 | 50.0 | 392.7 | 571.0 | 173.0 | 60.0 |
| T/T | 16 | 47,958.0 | 43,449.1 | −4508.8 | 43.8 | 242.3 | 313.5 | 71.2 | 68.8 |
| P | .015a | .006a | .39 | .77 | .086 | .013a | .21 | .76 | |
| CCL3 rs1130371 | |||||||||
| G/G | 28 | 58,307.0 | 59,695.6 | 1531.8 | 63.0 | 325.3 | 423.0 | 92.3 | 66.7 |
| G/A | 27 | 60,243.5 | 57,254.6 | −2988.9 | 33.3 | 369.6 | 539.1 | 169.5 | 55.6 |
| A/A | 2 | 74,195.5 | 79,523.5 | 5328.0 | 50.0 | 444.4 | 940.8 | 496.5 | 100 |
| P | .65 | .56 | .81 | .078 | .87 | .64 | .29 | .55 | |
| G/G | 28 | 58,307.0 | 59,695.6 | 1531.8 | 63.0 | 325.3 | 423.0 | 92.3 | 66.7 |
| Any A | 29 | 61,205.7 | 58,790.3 | −2415.3 | 34.5 | 374.8 | 566.8 | 192.0 | 58.6 |
| P | .64 | .91 | .60 | .060 | .65 | .23 | .32 | .59 | |
| Any G | 55 | 59,257.6 | 58,475.1 | −728.6 | 48.1 | 347.0 | 481.0 | 130.9 | 61.1 |
| A/A | 2 | 74,195.5 | 79,523.5 | 5328.0 | 50 | 444.4 | 940.8 | 496.5 | 100 |
| P | .38 | .30 | .77 | 1.00 | .74 | .70 | .17 | .52 | |
| CCL4 rs1634517 | |||||||||
| C/C | 32 | 56,542.2 | 60,138.3 | 3777.7 | 61.3 | 318.8 | 415.0 | 91.7 | 61.3 |
| C/A | 17 | 58,021.9 | 56,411.3 | −1610.6 | 41.2 | 392.7 | 623.8 | 231.1 | 70.6 |
| A/A | 8 | 76,479.8 | 61,677.9 | −14,801.9 | 12.5 | 387.4 | 548.3 | 160.9 | 50.0 |
| P | .085 | .88 | .24 | .036a | .80 | .29 | .46 | .64 | |
| C/C | 32 | 56,542.2 | 60,138.3 | 3777.7 | 61.3 | 318.8 | 415.0 | 91.7 | 61.3 |
| Any A | 25 | 63,928.4 | 58,096.6 | −5831.8 | 32.0 | 391.0 | 599.7 | 208.6 | 64.0 |
| P | .24 | .79 | .20 | .035a | .51 | .14 | .24 | 1.00 | |
| Any C | 49 | 57,055.6 | 58,818.3 | 1869.4 | 54.2 | 344.4 | 489.0 | 141.1 | 64.6 |
| A/A | 8 | 76,479.8 | 61,677.9 | −14,801.9 | 12.5 | 387.4 | 548.3 | 160.9 | 50.0 |
| P | .026a | .79 | .12 | .052 | .78 | .73 | .89 | .46 | |
| CCR5 rs1799988 | |||||||||
| T/T | 17 | 55,005.2 | 62,594.8 | 7589.5 | 70.6 | 288.4 | 436.2 | 147.8 | 70.6 |
| T/C | 21 | 60,885.6 | 56,854.4 | −3966.9 | 45 | 386.8 | 608.0 | 210.8 | 65.0 |
| C/C | 19 | 62,835.4 | 58,710.6 | −4124.8 | 31.6 | 365.7 | 435.9 | 70.2 | 52.6 |
| P | .59 | .82 | .36 | .071 | .75 | .39 | .48 | .54 | |
| T/T | 17 | 55,005.2 | 62,594.8 | 7589.5 | 70.6 | 288.4 | 436.2 | 147.8 | 70.6 |
| Any C | 40 | 61,811.8 | 57,758.7 | −4043.8 | 38.5 | 376.8 | 524.2 | 142.3 | 59.0 |
| P | .31 | .56 | .15 | .042a | .45 | .50 | .96 | .55 | |
| Any T | 38 | 58,254.9 | 59,491.9 | 1342.8 | 56.8 | 342.8147 | 529.0511 | 181.8 | 67.6 |
| C/C | 19 | 62,835.4 | 58,710.6 | −4124.8 | 31.6 | 365.7442 | 435.9084 | 70.2 | 52.6 |
| P | .49 | .92 | .49 | .095 | .84 | .40 | .29 | .38 | |
Abbreviations: SNP = single nucleotide polymorphism; VEGF-A = vascular endothelial growth factor A.
a
Statistically significant (P < .05). P values were calculated by Student’s unpaired t test and 1-way ANOVA for means of continuous measurements and Fisher’s exact test for change patterns in each cytokines.