Table 2.

Results of rRT-qPCR tests of materials collected by air samplers.

Sample ID	Approx. distance (m) from head of patient 1b	Approx. distance (m) from head of patient 2b	rRT-qPCR test	Cq value	SARS-CoV-2 genome equivalents/25 μL rRT-PCR test	SARS-CoV-2 genome equivalents/L of air
1-1 BioSpot	2	4.6	+	36.02	2.82E+03	94
1-2 BioSpot + HEPA	2	4.6	-	-	-	-
1-3 BioSpot	2	0 (PDb)	+	37.69	9.12E+02	30
2-1 VIVAS	4.8	3	+	37.42	1.15E+03	44
2-2 VIVAS+ HEPA	4.8	3	-	-	-	-
2-3 VIVAS	4.8	0 (PDd)	+	38.69	4.68E+02	16
SARS-CoV-2 vRNA	N/Ac	N/A	+	29.53	2.20E+05	N/A
N-genea DNA control - 1	N/A	N/A	+	26.56	1.00E+06	N/A
N-gene DNA control - 2	N/A	N/A	+	31.21	1.00E+05	N/A
N-gene DNA control - 3	N/A	N/A	+	34.71	1.00E+04	N/A
N-gene DNA control -4	N/A	N/A	+	37.74	1.00E+03	N/A
N-gene DNA control - 5	N/A	N/A	+	40.41	1.00E+02	N/A
N-gene DNA control - 6	N/A	N/A	+	-	1.00E+01	N/A
Known positive (NP swabe)	N/A	N/A	+	24.12	8.36E+06	N/A
Negative (no RNA) control	N/A	N/A	N/A	-	0	N/A

^aN-gene, N-gene plasmid (positive control template).

^bDistance from sampler inlet nozzle to patient’s head.

^cN/A, Not applicable.

^dPD, patient discharged.

^eNP, Nasopharyngeal swab from a person screened for SARS-CoV-2 at the UF EPI high-throughput COVID-19 research testing facility.