Table 1.
Summary of recovery rate and purity of exosome-like particles separated from MDA-MB-231 breast cancer cell culture media using the FerroChip. Atomic force microscopy characterization used a maximum ferret diameter of 150 nm to differentiate exosome-like particles and large extracellular vesicles, i.e., diameter of exosome-like particles is less than 150 nm, while diameter of large extracellular vesicles is larger than 150 nm. Super-resolution microscopy characterization used two different fluorescent colors to differentiate exosome-like particles and large extracellular vesicles. Recovery rate is defined as the number of exosome-like particles found in the side outlets over exosome-like particles found in all outlets of the FerroChip. Purity is defined as the number of exosome-like particles found in the side outlets over the sum of exosomes-like particles and large extracellular vesicles found in the side outlets of the FerroChip.
| Recovery rate | Purity | |
|---|---|---|
| AFM (n=1581) | 96.0% | 84.7% |
| Super-resolution microscopy (n=730) | 92.6% | 91.1% |
| Average of the above two methods | 94.3% | 87.9% |