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. Author manuscript; available in PMC: 2021 Aug 26.
Published in final edited form as: Lab Chip. 2020 Aug 26;20(17):3187–3201. doi: 10.1039/d0lc00609b

Table 1.

Summary of recovery rate and purity of exosome-like particles separated from MDA-MB-231 breast cancer cell culture media using the FerroChip. Atomic force microscopy characterization used a maximum ferret diameter of 150 nm to differentiate exosome-like particles and large extracellular vesicles, i.e., diameter of exosome-like particles is less than 150 nm, while diameter of large extracellular vesicles is larger than 150 nm. Super-resolution microscopy characterization used two different fluorescent colors to differentiate exosome-like particles and large extracellular vesicles. Recovery rate is defined as the number of exosome-like particles found in the side outlets over exosome-like particles found in all outlets of the FerroChip. Purity is defined as the number of exosome-like particles found in the side outlets over the sum of exosomes-like particles and large extracellular vesicles found in the side outlets of the FerroChip.

Recovery rate Purity
AFM (n=1581) 96.0% 84.7%
Super-resolution microscopy (n=730) 92.6% 91.1%
Average of the above two methods 94.3% 87.9%