Table 2.
Important mouse studies of microbiota-dependent phenotypes.
| Models used | Phenotype observed | Year | Controls used | Refs. |
|---|---|---|---|---|
| DSS-induced colitis | Nlrp6−/− mice had altered microbiota and increased susceptibility to colitis | 2011 | Co-housed controls, non-littermate controls | 16 |
| DSS-induced colitis and tumorigenesis | Nlrp6−/− mice were susceptible to colitis and tumorigenesis | 2011 | Non-littermate controls | 14 |
| DSS-induced colitis and tumorigenesis | Nlrp6−/− mice had enhanced colitis-induced tumor growth | 2011 | Non-littermate controls | 40 |
| Non-alcoholic fatty liver disease (NAFLD) and obesity | Co-housing WT mice with Nlrp6−/− mice increased the severity of NAFLD | 2012 | Co-housed controls, non-littermate controls | 42 |
| Azoxymethane-DSS-induced colitis and colorectal cancer | Nlrp6−/− mice were susceptible | 2013 | Co-housed controls, non-littermate controls | 43 |
| Enteritis | Nlrp6−/− mice were susceptible, Nlrp6 regulated goblet cell-mediated mucus secretion | 2014 | Co-housed controls, non-littermate controls | 11 |
| TLRs agonist-induced mucus secretion | Nlrp6 regulated TLR-induced mucus secretion by goblet cells | 2016 | Non-littermate controls | 53 |
| Microbiota analysis and colitis | No difference in microbiota composition and susceptibility to colitis was found between Nlrp6−/− or Asc−/− compared to that of WT mice | 2017 | Littermates control, non-littermate controls | 50 |
| Microbiota analysis | No difference in microbiota composition in a pathobiont-free condition. Introduction of pathobiont in an immunodeficient mice produced genotype-specific microbiota differences | 2017 | Pathobiont-free mice, non-littermate controls | 52 |
| Microbiota analysis and colitis | No difference in microbiota composition and susceptibility to colitis was observed between Nlrp6−/− and WT mice | 2017 | Littermate control animals | 51 |
| Microbiota analysis | Asc−/− mice had distinct microbiota composition compared to that of WT mice | 2017 | Littermate control animals | 45 |
| Analysis of inner mucus layer formation and function | Nlrp6 inflammasome did not regulate baseline inner mucus layer formation or function, but did regulate its function in response to TLRs agonist | 2019 | Littermates control, non-littermate controls | 54 |