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. 2020 Sep 15;51:118. doi: 10.1186/s13567-020-00843-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Screening of PRV open reading frames (ORFs) that modulate the cGAS–STING-induced transactivation of the IFN-β promoter. PK15 cells were transfected with IFN-β-Luc (200 ng), pCMV-RL (2 ng) mixed with pcDNA4-HA-cGAS (50 ng), pcDNA3-Flag-STING (20 ng), pcDNA3-Flag (200 ng), or pcDNA3-Flag expressing PRV ORFs (200 ng). The total amount of DNA was normalized using the pcDNA3-Flag and pcDNA4-HA empty vectors (E.V) in the negative control. The cells were collected 30 h post-transfection and then analysed for luciferase activity. The fold change in relative luciferase activity is calculated as the luciferase activity induced by cGAS–STING with or without each PRV ORF divided by that induced in the pcDNA3-Flag control group.