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. 2020 Sep 15;51:118. doi: 10.1186/s13567-020-00843-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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UL13 suppresses the expression of ISGs induced by cGAS–STING or poly(I:C). A PK15 cells were transfected with a mixture of pcDNA4-HA-cGAS (50 ng) and pcDNA3-Flag-STING (20 ng), together with pcDNA3-Flag or pcDNA3-Flag-UL13 (200 ng), for 30 h. The total amount of DNA was normalized to the corresponding empty vectors. The mRNA expression levels of ISGs were determined by semi-quantitative RT-PCR. B The experiment was performed as in (A), except that the PK15 cells were transfected with pcDNA3-Flag or pcDNA3-Flag-UL13 (500 ng) for 24 h, and then either treated with poly(I:C) (10 μg/mL) for 9 h or not.