Figure 1.

PARP1 depletion boosts the induction of MyoD target genes during differentiation. (a) Western blot analysis of PARP1 expression in C2.7 cell pools stably transduced with shPARP1 or with the empty vector (CTRL). Proteins were extracted from cells proliferating in growth medium (GM) or kept for 24 and 48 h in differentiation medium (DM 24 h and DM 48 h respectively. ERK1 was used as an invariant control. One representative experiment of two independent ones is shown. Full length blots are presented in Fig. S8. (b) RT-qPCR analysis of p57, myogenin (MyoG), p21 and Mef2C expression in cells cultured as described in (a). Expression levels, relative to those of Tbp RNA, are reported as the mean ± SEM of six independent experiments. Statistical significance: p-value < 0.05 (*) and < 0.01 (**).