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. 2020 Sep 15;10:15086. doi: 10.1038/s41598-020-72155-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PARP1 interacts with the regulatory regions of the MyoD target genes p57 and myogenin. ChIP-qPCR analysis for PARP1 (a) and MyoD (b) binding to KvDMR1, myogenin promoter (MyoG prom) and Rhodopsin promoter (Rho prom), used as a negative control, during C2.7 cell differentiation. Samples were prepared from undifferentiated cells proliferating in growth medium (GM), and from differentiated cells kept for 24 or 48 h in differentiation medium (DM 24 h and DM 48 h respectively) and immunoprecipitated either with the protein specific antibodies (IP samples) or with control IgG. Protein binding to the indicated regions is expressed as percentage of input. The mean ± SEM of four independent experiments is reported for PARP1 ChIP. Statistical significance: p-value < 0.05 (*) and < 0.01 (**). A representative experiment for MyoD ChIP is shown, with error bars corresponding to the mean of each sample analyzed in triplicate.