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. 2020 Sep 15;10:15086. doi: 10.1038/s41598-020-72155-8

Figure 8.

Figure 8

PARP1 re-expression impairs H3K4me3 accumulation at p57 and myogenin regulatory regions. ChIP-qPCR analysis for H3K4me3 association to KvDMR1 and myogenin promoter. shPARP1 cells stably re-expressing PARP1 (PARP1) and from shPARP1 control cells (CTRL) were collected 24 h after the shift to differentiation medium and samples were immunoprecipitated either with the H3K4me3 antibody (IP samples) or with the control IgG. Values are expressed as percentages of input and represent the mean ± SEM of three independent experiments. Statistical significance: p-value < 0.05 (*) and < 0.01 (**).