Table 2.
Cycling parameters for the first step PCR.
| Denaturation | 2 min | 95 °C | |
|---|---|---|---|
| Denaturation | 15 s | 94 °C | 10 cycles |
| Annealing | 30 s | 53.3 °C | |
| Elongation | 1 min/kb | 68 °C |
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