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. 2020 Jun 25;123(6):1024–1032. doi: 10.1038/s41416-020-0951-2

Fig. 3. ERG exon 4 SSOs affect VCaP cancer cell behaviour and signalling.

Fig. 3

a Representative immunofluorescence images for Ki67 (grey) and Hoechst (blue) after 48 h of 8 µM E4 SSO treatment in VCaP cells. b Quantification of Ki67+ VCaP cells after 48–96 h of 8 µM E4 SSO treatment (n = 3 for all time points). c Western blotting and quantification for regulators of cell cycle progression, cyclin D1 and c-Myc, following 72 h of 8 µM E4 SSO treatment in VCaP cells (n = 3). β-actin was used as loading control. d Caspase-3/7 staining of VCaP cells treated with E4 SSOs at 8 µM for 48 h and 96 h (n = 3 at all timepoints). e Quantification of VCaP cells migrated in transwell assays after 48 h of E4 SSO treatment at 8 µM (n = 4, except for untreated where n = 3). f and g Representative western blotting for and quantification of key components of the canonical Wnt signalling pathway (β-catenin and p-LRP6) following 72 h of SSO treatment in VCaP cells (8 µM) and MG63 (3 µM) cells respectively (n = 3). β-actin was used as loading control. h TopFlash assays to assess Wnt pathway activity following 72 h of SSO treatment in VCaP cells and MG63 cells. *** = p < 0.001, ** = p < 0.01, * = p < 0.05. Unt untreated; Ctrl SSO control SSO. Scale bar = 40 µm.