Table 2.
Oligonucleotides used in this study.
| Name | Sequence (5′ 3′) | Purpose |
|---|---|---|
| OHS767 | TCGAAGAGTTTGCCCCACAGC | 5′ flanking of mcrA |
| OHS769 | GGCTTTGGCCTGTATCATGACTTCA CATTGGAAGGTCGGGGAGCAG | 3′ mcrA with AfupyrG tail |
| OHS770 | TTTGGTGACGACAATACCTCCCGAC CCATCTTCAATGCCCAATATGCTC | 5′ mcrA with AfupyrG tail |
| OHS768 | AGCACTGTGGATGACAGCTCAAC | 3′ flanking of mcrA |
| OHS771 | CGACCCCAACTCTACCAGGACTC | 5′ nest of mcrA |
| OHS772 | CAATCGCTCTAACTGTCTACTCGCG | 3′ nest of mcrA |
| OMK607 | TCACATCTCGATGATTGGTTGAATG | 5′ flanking of flbA |
| OMK613 | GCTTTGGCCTGTATCATGACTTCA TGGCATTGAAGAGTGCAGGTCGGAG | 3′ flbA with AfupyrG tail |
| OMK614 | ATCGACCGAACCTAGGTAGGGTA ACAGTAATTATCTACACGCGTGATG | 5′ flbA with AfupyrG tail |
| OMK610 | ACTACTCACTACCTAACTTGACTG | 3′ flanking of flbA |
| OMK611 | TGGTTGAATGGTGTATGGGTCAGC | 5′ nest of flbA |
| OMK612 | TGTAGCTTTCGTTCAGGCGATAGTG | 3′ nest of flbA |
| OMK608 | ACTTCTGCAGTCGGAATTGGCCTG TGGCATTGAAGAGTGCAGGTCGGAG | 3′ flbA with AnipyroA tail |
| OMK609 | TGGTGAGAACACATGCACAACTTG ACAGTAATTATCTACACGCGTGATG | 5′ flbA with AnipyroA tail |
| OHS875 | ATATGAATTCATGTCGAACAATCCGAACCCG | 5′ mcrA_EcoRI |
| OHS878 | ATATGCGGCCGCCTGACCCAATCCACGGCGGT | 3′ mcrA _NotI |
| OHS657 | ATATGAATTCGGTGTAATTCTGGGTGTCTTGG | 5′ mcrA for C’_EcoRI |
| OMK589 | GCTGAAGTCATGATACAGGCCAAA | 5′ AfupyrG marker |
| OMK590 | ATCGTCGGGAGGTATTGTCGTCAC | 3′ AfupyrG marker |
| ONK395 | ATCTCATGGGTGCTGTGCGAAAGG | 5′ AnipyroA marker |
| ONK396 | TTGCATCGCATAGCATTGCATTGC | 3′ AnipyroA marker |
| OMK578 | CTGGCAGGTGAACAAGTC | 5′ brlA probe |
| OMK579 | AGAAGTTAACACCGTAGA | 3′ brlA probe |
| OHS0044 | GTAAGGATCTGTACGGCAAC | 5′ actin RT_probe |
| OHS0045 | AGATCCACATCTGTTGGAAG | 3′ actin RT_probe |
| OHS0578 | TGAGGAATTGACCACCGACA | 5′ fksA RT_probe |
| OHS0579 | GCACCAAGGATAGCAACAGG | 5′ fksA RT_probe |
| OHS0599 | GCGCGAAGAAGACTTCAAC | 5′ aflR RT_probe |
| OHS0600 | TGCAATAACTGCCGACGAC | 3′ aflR RT_probe |
aTail sequence is in italic.
bRestriction enzyme site is in bold.