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. 2020 Sep 16;5:194. doi: 10.1038/s41392-020-00232-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Influence of siRNA-PI3K ± CEPO on the phenotype and function of immune cells. a Western blot analysis of the efficiency of PI3K inhibition in DC and CD4⁺T cells after 48 h of cell transfection. b Flow cytometric analysis of the percentages of cell population with positive expression for CD11b, CD80, CD86, or MHC-II in DCs treated with siRNA control, siRNA control + CEPO, siRNA-PI3K, or siRNA-PI3K+CEPO, as indicated. c The influence of siRNA-PI3K and CEPO on the Th1, Th2, and Th1/Th2 ratio were determined by flow cytometry. d, e qPCR and western blot analysis of mRNA and protein level expression of IL-2, IFN-γ, TNF-α, IL-4, IL-5, IL-10, and IL-13 in CD4⁺ T cells treated with siRNA control, siRNA control + CEPO, siRNA-PI3K, or siRNA-PI3K+CEPO. GAPDH was used to normalize each protein expression. Images shown are representative of at least three independent experiments, data are expressed as mean ± SEM (*p < 0.05, p values were calculated by Student’s t-test)