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. 2020 Sep 16;5:194. doi: 10.1038/s41392-020-00232-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Influence of CEPO/PI3K/AKT on DC, Th1, Th2, Th17, and Treg and expression of MHC-II, Gr-1 and Foxp3. a Flow cytometric analysis of percentages of DC, Th1, Th2, Th17, and Treg in peripheral blood of rats from different groups (21 days after surgery): sham surgery, renal transplant alone, renal transplant + CEPO, renal transplant + EPOR blocker + CEPO, and renal transplant + LY292004 + CEPO. b Immunohistochemistry analysis of the protein expression of MHC-II, Gr-1, and Foxp3 in the kidney tissues from different groups (21 days after surgery): sham-surgery, renal transplant alone, renal transplant + CEPO, renal transplant + EPOR blocker + CEPO, and renal transplant + LY292004 + CEPO. Scale bar, 100 µm. c Immunohistochemistry staining scores for MHC-II, Gr-1, and Foxp3 in different groups. The representative images of each group from five samples were shown. Data are expressed as mean ± SEM (*p < 0.05, p values were calculated by Student’s t-test)