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. 2020 Sep 15;10:15145. doi: 10.1038/s41598-020-71618-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Electroporation efficiency and survival of B. dendrobatidis at 750 V (green), 1000v (blue), and 1250 V (purple). (A) Representative images of cells after electroporation at 1000v showing live cells adhered to the slide and attempting amoeboid movement. (B) Representative flow cytometry data from a single replicate showing the fluorescence intensity of single cell events for non-electroporated, no dextran controls (grey); non-electroporated, dextran incubated controls (red); and electroporated, dextrans incubated treatments (colors vary by voltage). (C) Percent of single cell events with fluorescence intensities above the non-electroporated, no dextran control relative to all single cell events for three independent biological replicates. (D) Percent survival of cells in electroporated, dextran incubated controls in three independent biological replicates. Counts were normalized to the respective non-electroporated, no dextran controls.