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. 2020 Sep 15;10:15145. doi: 10.1038/s41598-020-71618-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Electroporation efficiency of S. punctatus for 750 V (green), 1000 V (blue), and 1250 V (purple). (A) Representative cells from a single replicate at 1000 V showing positive cells with dextran inside the cell and not stuck to the cell wall/coat. (B) Representative flow cytometry data from a single replicate showing the fluorescence intensity of single cell events for non-electroporated, no dextran controls (grey); non-electroporated, dextran incubated controls (red); and electroporated, dextrans incubated treatments (colors vary by voltage). (C) Percent of single cell events with fluorescence intensities above the non-electroporated, no dextran control relative to all single cell events for three independent biological replicates.