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. 2020 Sep 15;11:4632. doi: 10.1038/s41467-020-18422-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Eight Brainbow labeled PV neurons reconstructed with nTracer. Thick neurites represent dendrites; thin neurites represent axons. The neuron IDs are overlaid. b Same as a, plus all of the other 189 innervating axons overlaid and 422 identified synapses marked with white circles. c Connectivity matrix between the eight reconstructed PV neurons. d Connectivity matrix as in c, plus the 189 innervating axons. e Plot of the maximum and minimum number of unique presynaptic PV neurons that innervate each of the eight postsynaptic PV neurons. Circles represent the number of spatially distinct PV axons that formed synapses, while triangles represent the number of unique colors that could be identified from the innervating axons. f Distribution of the number of soma targeting and dendrite targeting axons as a function of the number of synapses they form. Each of the eight afferent PV neurons are color coded and plotted with the average represented by the bar graph. g Total number of somatic inhibitory synapses were plotted relative to the PV to PV subset for each of the eight postsynaptic PV neurons. h Inhibitory PSD (Gephyrin) size plotted for all the PV to PV synapses found on each of the eight postsynaptic PV neurons. Scale bars: 10 μm (pre-expansion size).