Table 2.
Dissociation constants (Kd in nM, average ± SD, n = 2) for HUSpm wild type and HUSpm bearing mutations R57A, K80A and K88A binding to various DNA structures calculated from EMSA experiments (see Fig. 5B legend for a description of the DNA sequences).
| DNA | HUSpm wild type | HUSpm_R57A | HUSpm_K80A | HUSpm_K88A |
|---|---|---|---|---|
| Nick (N) | 5.2 ± 1.1 | 398 ± 45 | 69 ± 20 | 220 ± 40 |
| DS48 (48) | 18.6 ± 4.8 | 788 ± 100 | 163 ± 27 | 390 ± 55 |
| A1 bulge (A1) | 2.5 ± 0.6 | 175 ± 25 | 41 ± 8 | 83 ± 15 |
| A7 bulge (A7) | 2.0 ± 0.5 | 121 ± 15 | 13 ± 3 | 51 ± 8 |
| DS24a (24a) | 2.6 ± 0.6 | 230 ± 30 | 40 ± 10 | 84 ± 20 |