Table 2.

In situ gene expression (Mean ± SD) of Th17 signature and associated molecules in CD3⁺CD4⁺ T cells isolated from 48 h MLSA stimulated PBMCs.

S. no	Gene	Accession number	Gene expression (ΔCt) Mean ± SD		P value
			T1R (19)	NR(19)
1	IL-1β	Hs.126256	2.25 ± 1.3	2.97 ± 1.3	ns
2	Stat3	Hs.463059	2.55 ± 0.8	2.97 ± 0.6	p < 0.04
3	TNF-α	Hs.241570	3.91 ± 1.3	4.62 ± 0.8	ns
4	CCL22	Hs.534347	3.13 ± 1.0	4.82 ± 1.2	p < 0.0001
5	RORC	Hs.256022	3.98 ± 2.1	7.07 ± 2.2	p < 0.0001
6	JAK1	Hs.207538	2.04 ± 0.6	2.62 ± 0.3	p < 0.005
7	IL-2	Hs.89679	4.29 ± 1.7	8.12 ± 3.6	p < 0.0003
8	IL-17F	Hs.272295	4.1 ± 1.8	8.0 ± 3.3	p < 0.0001
9	JAK2	Hs.656213	2.77 ± 1.1	3.92 ± 0.9	p < 0.001
10	CCL20	Hs.75498	3.54 ± 1.6	6.42 ± 2.1	p < 0.0001
11	IL-17D	Hs.655142	6.8 ± 2.5	10.8 ± 2.7	p < 0.0001
12	IL-23A	Hs.98309	3.38 ± 1.7	6.22 ± 1.7	p < 0.0001
13	IL-17A	Hs.41724	4.68 ± 2.6	9.02 ± 4.1	p < 0.0002
14	IL-23R	Hs.677426	4.01 ± 2.9	7.87 ± 3.7	p < 0.0005
15	IL-10	Hs.193717	3.32 ± 1.4	4.77 ± 1.0	p < 0.0005
16	IL-17C	Hs.278911	4.87 ± 2.1	7.77 ± 2.7	p < 0.0006
17	IL-6R	Hs.709210	2.95 ± 1.0	3.72 ± 0.6	p < 0.003
18	IL-6	Hs.654458	3.79 ± 1.5	6.82 ± 2.3	p < 0.0001
19	IL-25	Hs.302036	5.26 ± 2.6	9.52 ± 3.5	p < 0.0002
20	IL-21	Hs.567559	3.64 ± 2.5	7.32 ± 3.1	p < 0.0003
21	IL-22	Hs.287369	3.45 ± 2.7	8.02 ± 4.0	p < 0.0001

Classification of patients according to Ridley–Jopling^25,26 as in legend to Table 1.*p < 0.01, **p < 0.001, ***p < 0.0001 by two tailed Mann–Whitney test. P < 0.05 was considered significant, ns = non significant.