Table 1.
Statistics of the PacBio sequencing data, ROI databases and ICE clustering
| cDNA library | 1-6 KB |
|---|---|
| PacBio sequencing | |
| polymerase reads | 720,832 |
| Reads of insert | 658,323 |
| Read bases of insert | 1,505,553,284 |
| Mean read length of insert | 2286 |
| Mean read quality of insert | 0.94 |
| Mean number of passes | 12 |
| Date size (GB) | 19.82 |
| ROI databases | |
| Number of five prime reads | 506,205 |
| Number of three prime reads | 521,059 |
| Number of poly-A reads | 514,174 |
| Number of filtered short reads | 15,350 |
| Number of non-full-length reads | 193,513 |
| Number of full-length reads | 449,460 |
| Number of full-length non-chimeric reads | 441,885 |
| Average full-length non-chimeric read length | 1969 |
| ICE clustering | |
| Number of consensus isoforms | 227,516 |
| Average consensus isoforms read length | 2086 |
| Number of polished high-quality isoforms | 148,836 |
| Number of polished low-quality isoforms | 78,366 |
| Percent of polished high-quality isoforms (%) | 65,42 |
| Non-redundant transcripts | 80,654 |
KB (kilobase): A commonly used length unit for DNA, indicating one thousand base pairs. GB: A unit used to measure the amount of data, where 1 GB = 1,000,000 bp.