Fig. 3. Image data and tissue parameter identification process.
A) Top row from left to right: fresh tumor surface in gel with inserted metal pins, BPD fluorescence, stiffness map. Bottom row: Masson’s Trichrome staining, lectin fluorescence, segmented collagen map from MT staining. All images are co-registered to MT staining data. B) Average stiffness detected from spleen (4.0±0.3 kPa) and pancreas (2.3±0.3 kPa). These regions are excluded from tumor analysis by manual ROI drawn on pathology data.