Mortality Risk Profiling of Staphylococcus aureus Bacteremia by Multi-omic Serum Analysis Reveals Early Predictive and Pathogenic Molecular Signatures

. Author manuscript; available in PMC: 2021 Sep 3.

Published in final edited form as: Cell. 2020 Sep 3;182(5):1311–1327.e14. doi: 10.1016/j.cell.2020.07.040

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Lysyl Endopeptidase (LysC)	Wako Labs	129–02541
Sequencing-grade Trypsin	Promega Corporation	V5113
Anhydrous Acetonitrile	Sigma-Aldrich	271004
Tandem Mass Tags (TMT)	Thermo Fisher	90110
50% Hydroxylamine	Aldrich Chemistry	467804
C₄ 5 μm Stationary Phase	Sepax	109045–0000
C₁₈ 3 μm Stationary Phase	Sepax	101183–0000
C₁₈ 1.8 μm Stationary Phase	Sepax	101181–0000
Critical Commercial Assays
Pierce™ Quantitative Colorimetric Peptide Assay	Thermo Fisher	23275
Fetuin-B ELISA Kit	RayBiotech	ELH-FetuinB-1
IGFBP3 ELISA Kit	RayBiotech	ELH-IGFBP3–1
SERPIND1 ELISA Kit	RayBiotech	ELH-SERPIND1–1
Deposited Data
Standard Proteomics	ProteomeXchange	PXD018030
PTM Proteomics	ProteomeXchange	PXD018031
Metabolomics	MassIVE	MSV000083593
Experimental Models: Organisms/Strains
Mouse: 8-week old female CD1	Charles River	CD1
Software and Algorithms
Proteome Discoverer (2.1)	Thermo Fisher	N/A
R Studio (1.1.463)	R Studio	N/A
Prism (7.0b)	GraphPad	N/A
Cytoscape (3.7.2)	(Shannon et al., 2003)	N/A
Ingenuity Pathway Analysis (01.16)	Qiagen	N/A
Other
Sep-Pak Cartridge 1 cc	Waters	WAT054960
HPLC Column	Thermo Fisher	720105–254630
Fused Silica Capillary Tubing	Polymicro Technologies	106815–0023
Orbitrap Fusion Tribrid Mass Spectrometer	Thermo Fisher	IQLAAEGAAPFADBMBCX