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. Author manuscript; available in PMC: 2021 Aug 6.
Published in final edited form as: Cell Stem Cell. 2020 Jul 15;27(2):326–335.e4. doi: 10.1016/j.stem.2020.06.018

Figure 1. DNMT3A mutant cells dominates the blood without increase in mutation number or spectrum.

Figure 1.

(A) Variant allele frequency (VAF) of DNMT3A c.2312G>A substitution (DNMT3AR771Q) measured by amplicon sequencing. 3 separate peripheral blood measurements were taken at the indicated years. (B) Summary of the number of base substitutions (covered ≥3X) for WT and LCL clones (C) Mutational signatures for WT and MUT clones that comprised at least 10% of the total signature classification. (D) Top: phylogenetic tree of the LCL clones constructed by identifying shared mutations. Branch lengths are proportional to the number of somatic mutations unique to each clone. DNMT3A-MUT clones are red. Bottom: zoom in of the x-axis (E) Phylogenetic tree of the LCL clones reflecting their representation in the PB with the VAF of key mutations indicated on the branches.