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. 2020 Sep 3;16(9):e1008773. doi: 10.1371/journal.ppat.1008773

Fig 4. Inhibitory effects of NS5 on IFN-α and β expression.

Fig 4

DEF were transfected with plasmids for expression of Flag-NS5(GI) or Flag-NS5(GIII) at amounts of 250, 500, and 1,000 ng and incubated for 24 h. The transfectants were mock-treated (-poly(I:C)) or treated with poly(I:C) (+poly(I:C)) for 12 h. (A) The expression of Flag-NS5(GI) and Flag-NS5(GIII) in the transfectants was detected by western blotting with anti-Flag antibodies. (B) The mRNA levels of IFN-α and β in the cell pellets were determined by using qRT-PCR. (C) The concentrations of IFN-α and β proteins in the supernatants were determined by using ELISA. All data are presented as mean ± SD from three independent experiments. **, p < 0.01; *, p < 0.05; ns, no significant difference, by Student’s t-test.