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. 2020 Sep 3;16(9):e1008773. doi: 10.1371/journal.ppat.1008773

Fig 7. Effects of NS5-V372A and NS5-H386Y variations on IFN-α and β induction.

Fig 7

(A) Schematic diagram of substitution mutant viruses used in this study. Viral proteins of GI and GIII strains are highlighted in blue and gray, respectively. (B) DEF were infected with the indicated substitution mutant viruses at 1 MOI and harvested at 24 hpi for measurement of IFN-α and β production at the mRNA level by qRT-PCR. (C and D) DEF were transfected with the indicated plasmids and incubated for 24 h. The transfectants were mock-treated (-poly(I:C)) or treated with poly(I:C) (+poly(I:C)) for 12 h. The mRNA levels of IFN-α and β in the cell pellets were determined by using qRT-PCR (C). The expression of Flag-NS5 and Flag-NS5 deletion mutants were analyzed with western blotting with anti-Flag antibodies (D). All data are presented as mean ± SD from three independent experiments. **, p < 0.01; *, p < 0.05; ns, no significant difference, by Student’s t-test.